{"id":121056,"date":"2025-01-28T21:15:09","date_gmt":"2025-01-28T21:15:09","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2025\/01\/28\/conseils-pour-le-reconditionnement-en-vue-dun-essai-elisa\/"},"modified":"2025-01-28T21:34:41","modified_gmt":"2025-01-28T21:34:41","slug":"elisa","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2025\/01\/28\/elisa\/","title":{"rendered":"Reconditionnement des embouts pour un essai ELISA"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb2&Prime; bottom_padding=\u00a0\u00bb2&Prime; back_color=\u00a0\u00bbcolor-366045&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb224020&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb2\/3&Prime; uncode_shortcode_id=\u00a0\u00bb122956&Prime;][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1&Prime; text_size=\u00a0\u00bbfontsize-155944&Prime; text_weight=\u00a0\u00bb600&Prime; text_space=\u00a0\u00bbfontspace-781688&Prime; uncode_shortcode_id=\u00a0\u00bb574267&Prime; text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]<span class=\"font-100719 font-377187\">Reconditionnement des embouts pour un essai ELISA<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1&Prime;][vc_button button_color=\u00a0\u00bbcolor-118531&Prime; radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0&Prime; link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb988730&Prime;]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; back_image=\u00a0\u00bb121065&Prime; parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/3&Prime; uncode_shortcode_id=\u00a0\u00bb702909&Prime;][vc_single_image media=\u00a0\u00bb119266&Prime; media_width_percent=\u00a0\u00bb100&Prime; alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb173810&Prime;][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/1&Prime;][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb744229&Prime;]Cette \u00e9tude a compar\u00e9 les performances des pointes de pipettes neuves et reconditionn\u00e9es dans un test ELISA pour le TNF-\u03b1 humain. Les pointes P1000 et P200 ont \u00e9t\u00e9 contamin\u00e9es avec un standard reconstitu\u00e9 de TNF-\u03b1 humain, puis reconditionn\u00e9es \u00e0 l\u2019aide de notre proc\u00e9d\u00e9. Cinq standards ont \u00e9t\u00e9 pr\u00e9par\u00e9s conform\u00e9ment au sch\u00e9ma de dilution du fabricant (Millipore, EZHTNFA-150K). Le test a suivi les \u00e9tapes d\u2019incubation et d\u2019ajout de r\u00e9actifs sp\u00e9cifi\u00e9es dans le <a href=\"https:\/\/www.sigmaaldrich.com\/deepweb\/assets\/sigmaaldrich\/product\/documents\/324\/626\/protocol-ezhtnfa150k-ms.pdf\">protocole<\/a> associ\u00e9. Les lectures d\u2019absorbance ont \u00e9t\u00e9 mesur\u00e9es \u00e0 450 nm et 590 nm, et la densit\u00e9 optique (DO) a \u00e9t\u00e9 calcul\u00e9e comme la diff\u00e9rence d\u2019absorbance entre les deux longueurs d\u2019onde. Des courbes standards ont \u00e9t\u00e9 \u00e9tablies pour les pointes neuves et reconditionn\u00e9es afin de comparer et analyser leurs lectures d\u2019absorbance et leurs tendances.<\/p>\n<p>De plus, des \u00e9chantillons de contr\u00f4le qualit\u00e9 ont \u00e9t\u00e9 inclus pour valider l\u2019efficacit\u00e9 et la fonctionnalit\u00e9 du test. Les courbes standards g\u00e9n\u00e9r\u00e9es ont \u00e9t\u00e9 utilis\u00e9es pour d\u00e9terminer les concentrations de ces contr\u00f4les, permettant une comparaison des performances des pointes neuves et reconditionn\u00e9es pour des concentrations standard inconnues.<\/p>\n<p>Cette exp\u00e9rience nous a permis d\u2019\u00e9valuer la pr\u00e9cision des pointes reconditionn\u00e9es dans un test ELISA.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/2&Prime;][vc_single_image media=\u00a0\u00bb121051&Prime; media_width_percent=\u00a0\u00bb100&Prime; uncode_shortcode_id=\u00a0\u00bb122332&Prime;][vc_single_image media=\u00a0\u00bb121049&Prime; media_width_percent=\u00a0\u00bb100&Prime; uncode_shortcode_id=\u00a0\u00bb109965&Prime;][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50&Prime; radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime; uncode_shortcode_id=\u00a0\u00bb117897&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb120655&Prime; media_size=\u00a0\u00bb70&Prime; text_size=\u00a0\u00bbh2&Prime; align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb191112&Prime;]Les courbes standards obtenues avec les pointes neuves et reconditionn\u00e9es pr\u00e9sentaient des \u00e9quations de tendance tr\u00e8s similaires. De plus, les concentrations des contr\u00f4les de qualit\u00e9 d\u00e9termin\u00e9es \u00e0 partir des courbes standards pour les pointes neuves et reconditionn\u00e9es \u00e9taient comparables et se situaient dans la plage sp\u00e9cifi\u00e9e par le kit. Cela indique que les pointes reconditionn\u00e9es ne contenaient aucun r\u00e9sidu de standard.[\/vc_icon][vc_icon icon_image=\u00a0\u00bb120653&Prime; media_size=\u00a0\u00bb70&Prime; text_size=\u00a0\u00bbh2&Prime; align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbConclusion\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb311798&Prime;]Notre proc\u00e9d\u00e9 fonctionne de mani\u00e8re \u00e0 permettre l&rsquo;utilisation des pointes reconditionn\u00e9es dans des exp\u00e9riences hautement sensibles, telles qu&rsquo;un test ELISA.[\/vc_icon][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb2&Prime; bottom_padding=\u00a0\u00bb2&Prime; back_color=\u00a0\u00bbcolor-366045&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb224020&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2&Prime; overlay_alpha=\u00a0\u00bb50&Prime; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[166],"tags":[],"class_list":["post-121056","post","type-post","status-publish","format-standard","hentry","category-validation-fr"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121056","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/comments?post=121056"}],"version-history":[{"count":15,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121056\/revisions"}],"predecessor-version":[{"id":121075,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121056\/revisions\/121075"}],"wp:attachment":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/media?parent=121056"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/categories?post=121056"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/tags?post=121056"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}