{"id":121004,"date":"2024-10-15T21:15:33","date_gmt":"2024-10-15T21:15:33","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2024\/10\/15\/culture-de-labattage\/"},"modified":"2024-10-15T21:30:00","modified_gmt":"2024-10-15T21:30:00","slug":"culture-cellulaire","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2024\/10\/15\/culture-cellulaire\/","title":{"rendered":"Culture cellulaire"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb605952″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb113011″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb119339″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Culture cellulaire<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb824723″]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb119296″ back_position=\u00a0\u00bbright top\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb746348″][vc_single_image media=\u00a0\u00bb119266″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb318485″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb511556″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb360120″][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb146312″]Passage et ensemencement des cellules[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb112047″]Cette \u00e9tude a \u00e9valu\u00e9 les performances de divers consommables de laboratoire neufs et reconditionn\u00e9s (jusqu’\u00e0 cinq cycles de reconditionnement). Ces consommables comprenaient des flacons T-75 trait\u00e9s pour la culture cellulaire, des bo\u00eetes de P\u00e9tri* et des plaques \u00e0 12 puits trait\u00e9es pour la culture cellulaire. Pour comparer les consommables neufs et reconditionn\u00e9s, ils ont \u00e9t\u00e9 ensemenc\u00e9s avec des cellules Rat-1 \u00e0 une concentration de 10^4 cellules\/mL. Chaque consommable a \u00e9t\u00e9 test\u00e9 en double afin de tenir compte de toute contamination potentielle. Au cours de chaque cycle de reconditionnement, les bo\u00eetes de Petri neuves et les consommables reconditionn\u00e9s ont \u00e9t\u00e9 ensemenc\u00e9s et incub\u00e9s \u00e0 37,5\u00b0C. Les consommables reconditionn\u00e9s ont \u00e9t\u00e9 retir\u00e9s de l’incubateur apr\u00e8s 2 jours pour \u00e9valuer la confluence et la morphologie des cellules \u00e0 l’aide de photographies prises \u00e0 diff\u00e9rents grossissements (10X et 20X) et pour \u00e9valuer la viabilit\u00e9 des cellules \u00e0 l’aide d’un compteur de cellules automatis\u00e9. Apr\u00e8s avoir recueilli les donn\u00e9es, les consommables ont \u00e9t\u00e9 reconditionn\u00e9s selon notre proc\u00e9d\u00e9, puis r\u00e9ensemenc\u00e9s. Ce processus a \u00e9t\u00e9 r\u00e9p\u00e9t\u00e9 jusqu’\u00e0 cinq cycles de reconditionnement. Des comparaisons ont \u00e9t\u00e9 effectu\u00e9es entre la confluence des consommables reconditionn\u00e9s et les nouvelles bo\u00eetes de Petri ensemenc\u00e9es au m\u00eame moment. Le diam\u00e8tre moyen des cellules a \u00e9t\u00e9 mesur\u00e9 pour les diff\u00e9rents consommables reconditionn\u00e9s et compar\u00e9 pour tous les cycles de reconditionnement. La m\u00eame chose a \u00e9t\u00e9 faite pour la viabilit\u00e9 des cellules.<\/p>\n

Cette exp\u00e9rience nous a permis d’\u00e9valuer la performance des consommables reconditionn\u00e9s et d’analyser les effets du reconditionnement sur la croissance cellulaire.<\/p>\n

* Aucune donn\u00e9e n’a \u00e9t\u00e9 collect\u00e9e lors du premier reconditionnement.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb162287″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb153986″][vc_single_image media=\u00a0\u00bb121008″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb182426″][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb133292″][vc_single_image media=\u00a0\u00bb121010″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb159672″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb144629″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb360120″][vc_single_image media=\u00a0\u00bb121014″ media_width_percent=\u00a0\u00bb81″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb181743″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb121955″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb872726″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb120655″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb178751″]Les consommables reconditionn\u00e9s ont g\u00e9n\u00e9ralement atteint une confluence cellulaire comparable \u00e0 celle des bo\u00eetes de Petri neuves ensemenc\u00e9es le m\u00eame jour. En outre, aucune tendance claire ou diminution stricte de la taille des cellules n’a \u00e9t\u00e9 observ\u00e9e entre les consommables neufs et reconditionn\u00e9s au cours des cycles de reconditionnement. Bien que la viabilit\u00e9 cellulaire ait fluctu\u00e9 au cours des cycles de reconditionnement, elle n’a pas montr\u00e9 de d\u00e9clin constant. Ces r\u00e9sultats indiquent que le processus de reconditionnement n’a pas eu d’effet n\u00e9gatif sur les performances des consommables.[\/vc_icon][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb451322″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb120653″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbConclusion\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb161462″]Notre proc\u00e9d\u00e9 a fonctionn\u00e9 de mani\u00e8re \u00e0 permettre l’utilisation de consommables reconditionn\u00e9s jusqu’\u00e0 cinq fois pour le passage et l’ensemencement de cellules.<\/p>\n

Cette conclusion s’applique aux consommables avec un traitement de surface chimique, \u00e0 l’exclusion de ceux avec un traitement de surface biologique, tels que les consommables recouverts de collag\u00e8ne.[\/vc_icon][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb605952″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[166,167],"tags":[],"class_list":["post-121004","post","type-post","status-publish","format-standard","hentry","category-validation-fr","category-etude-de-cas"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121004","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/comments?post=121004"}],"version-history":[{"count":8,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121004\/revisions"}],"predecessor-version":[{"id":121022,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/121004\/revisions\/121022"}],"wp:attachment":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/media?parent=121004"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/categories?post=121004"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/tags?post=121004"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}