{"id":120956,"date":"2024-09-09T16:12:37","date_gmt":"2024-09-09T16:12:37","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2024\/09\/09\/mesures-dabsorbance\/"},"modified":"2026-06-29T18:46:25","modified_gmt":"2026-06-29T18:46:25","slug":"mesures-dabsorbance","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2024\/09\/09\/mesures-dabsorbance\/","title":{"rendered":"Mesures d’absorbance"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb224020″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb122956″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb370023″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Mesures d’absorbance<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb988730″]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb120969″ parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb396494″][vc_single_image media=\u00a0\u00bb119635″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb173810″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ content_parallax=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb214359″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb133461″][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb187367″]Cuvettes reconditionn\u00e9es pour le test d’absorbance[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb185731″]<\/p>\n

Afin de d\u00e9terminer si le reconditionnement a une incidence sur les propri\u00e9t\u00e9s optiques des cuvettes semi-micro en polystyr\u00e8ne, les mesures d’absorbance ont \u00e9t\u00e9 compar\u00e9es entre des cuvettes neuves et des cuvettes reconditionn\u00e9es, et ce sur cinq cycles de reconditionnement.<\/p>\n

Quatre solutions ont \u00e9t\u00e9 test\u00e9es : trois concentrations de dichromate de potassium dans de l’acide perchlorique (20, 120 et 600 mg\/L), un \u00e9talon d’absorbance UV bien \u00e9tabli, ainsi qu’une solution aqueuse d’\u00e9osine Y, couramment utilis\u00e9e pour l’\u00e9valuation de la lin\u00e9arit\u00e9 dans le domaine du visible. Chaque solution a \u00e9t\u00e9 mesur\u00e9e \u00e0 sa longueur d’onde caract\u00e9ristique (350, 430 ou 517 nm). Apr\u00e8s chaque cycle de remise en \u00e9tat, cinq cuvettes par solution ont \u00e9t\u00e9 mises de c\u00f4t\u00e9 et test\u00e9es aux c\u00f4t\u00e9s de cinq cuvettes neuves servant de t\u00e9moins. Chaque cuvette a d’abord \u00e9t\u00e9 analys\u00e9e apr\u00e8s avoir \u00e9t\u00e9 remplie d’eau Milli-Q en tant que t\u00e9moin, puis remplie de la solution correspondante. <\/p>\n

Pour conna\u00eetre la m\u00e9thodologie compl\u00e8te, consultez notre \u00e9tude : Mansouri et al., 2026 \u2014 npj Materials Sustainability<\/em><\/a><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb115377″][vc_single_image media=\u00a0\u00bb121325″ media_width_percent=\u00a0\u00bb90″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb184139″][vc_column_text text_lead=\u00a0\u00bbsmall\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb372902″]R\u00e9sultats des mesures d’absorbance dans des<\/span><\/span>cuvettes semi-micro \u00e0 l\u2019aide de<\/span>fi<\/span>et Milli-<\/span>Q eau. R\u00e9sultats pour les concentrations de 20 mg\/L et 120 mg\/L <\/span>Les solutions de dichromate de potassium (PD) sont repr\u00e9sent\u00e9es <\/span>\u00e0 350 nm, tandis que la solution de PD \u00e0 600 mg\/L est <\/span>observ\u00e9e \u00e0 430 nm et la solution d\u2019\u00e9osine Y \u00e0 <\/span>517 nm.<\/span>[\/vc_column_text][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb130744″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon position=\u00a0\u00bbleft\u00a0\u00bb icon_image=\u00a0\u00bb120655″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb200880″][\/vc_icon][vc_column_text uncode_shortcode_id=\u00a0\u00bb210888″]Les cuvettes reconditionn\u00e9es ont permis d’obtenir des mesures d’absorbance comparables \u00e0 celles des cuvettes neuves dans toutes les conditions test\u00e9es. Aucune diff\u00e9rence statistiquement significative n’a \u00e9t\u00e9 constat\u00e9e entre les cuvettes neuves et les cuvettes reconditionn\u00e9es, que ce soit pour les mesures r\u00e9alis\u00e9es avec une solution ou avec de l’eau Milli-Q, et ce, pour l’ensemble des cinq cycles de reconditionnement. Les tailles d’effet variaient l\u00e9g\u00e8rement d’une condition \u00e0 l’autre, mais ne montraient aucune tendance constante en faveur de l’un ou l’autre groupe, et les \u00e9carts-types ne laissaient entrevoir aucune tendance directionnelle, ce qui indique que la variation observ\u00e9e refl\u00e8te probablement des facteurs inh\u00e9rents \u00e0 la spectrophotom\u00e9trie, tels que la pr\u00e9paration de la solution, la manipulation ou le moment de la mesure, plut\u00f4t qu’un quelconque effet du reconditionnement.[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

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