{"id":120405,"date":"2023-09-04T17:06:04","date_gmt":"2023-09-04T17:06:04","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2023\/09\/04\/presence-de-proteines\/"},"modified":"2026-06-29T19:42:21","modified_gmt":"2026-06-29T19:42:21","slug":"presence-de-proteines","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2023\/09\/04\/presence-de-proteines\/","title":{"rendered":"Quantification des prot\u00e9ines"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb605952″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb113011″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb205086″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Pr\u00e9sence de prot\u00e9ines<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb824723″]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb119642″ back_position=\u00a0\u00bbright top\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb746348″][vc_single_image media=\u00a0\u00bb119635″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb318485″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb511556″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb134650″][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb236418″]Microplaques et tubes reconditionn\u00e9s pour le dosage des prot\u00e9ines par la m\u00e9thode BCA de Pierce[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb711417″][vc_column_text uncode_shortcode_id=\u00a0\u00bb120693″]<\/p>\n

Afin de d\u00e9terminer si le reconditionnement influe sur la pr\u00e9cision de la quantification des prot\u00e9ines, nous avons r\u00e9alis\u00e9 le test BCA de Pierce selon deux m\u00e9thodes : une m\u00e9thode sur microplaque (plaques en polystyr\u00e8ne \u00e0 96 puits) et une m\u00e9thode sur tube (tubes de microcentrifugation en polypropyl\u00e8ne de 1,5 ml).<\/p>\n

Trois microplaques et 18 tubes ont \u00e9t\u00e9 soumis \u00e0 cinq cycles de reconditionnement et r\u00e9utilis\u00e9s pour r\u00e9aliser le dosage apr\u00e8s chaque cycle, en comparaison avec des consommables neufs \u00e0 chaque cycle. Les deux formats ont suivi les protocoles<\/a> BCA standard, avec 9 \u00e9talons de concentration et un blanc \u00e0 l’eau Milli-Q dans chaque condition. L\u2019absorbance a \u00e9t\u00e9 mesur\u00e9e \u00e0 562 nm, et des courbes d\u2019\u00e9talonnage ont \u00e9t\u00e9 \u00e9tablies pour chaque condition. L\u2019absorbance du blanc, l\u2019absorbance des courbes d\u2019\u00e9talonnage et les \u00e9carts-types ont \u00e9t\u00e9 compar\u00e9s entre les consommables neufs et reconditionn\u00e9s afin d\u2019\u00e9valuer tout effet sur la pr\u00e9cision et la variabilit\u00e9 des mesures. <\/p>\n

Pour conna\u00eetre la m\u00e9thodologie compl\u00e8te, consultez notre \u00e9tude : Mansouri et al., 2026 \u2014 npj Materials Sustainability<\/em><\/a><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb117024″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb190872″][vc_single_image media=\u00a0\u00bb121331″ media_width_percent=\u00a0\u00bb90″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb687528″][vc_column_text text_lead=\u00a0\u00bbsmall\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb187262″]Quantification des prot\u00e9ines <\/span><\/span>dans des microplaques \u00e0 96 puits <\/span>et des micro-tubes \u00e0 l’aide du test BCA. L’analyse statistique a \u00e9t\u00e9 <\/span><\/span>r\u00e9alis\u00e9e \u00e0 l’aide de la m\u00e9thode de Mann<\/span>\u2013<\/span>Whitney U avec correction de Bonferroni-Holm. <\/span><\/span><\/span>Aucune diff\u00e9rence statistiquement significative<\/span>fi<\/span>n’a \u00e9t\u00e9 observ\u00e9e. Chaque \u00e9talon a \u00e9t\u00e9 test\u00e9 en 5 <\/span> r\u00e9plicats<\/span> <\/span><\/span>par plaque pour les microplaques et en trois r\u00e9plicats pour les tubes (soit un total de <\/span><\/span><\/span>18 tubes).<\/span><\/span><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb121955″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb872726″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon position=\u00a0\u00bbleft\u00a0\u00bb icon_image=\u00a0\u00bb120655″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb177936″][\/vc_icon][vc_column_text uncode_shortcode_id=\u00a0\u00bb157919″]<\/p>\n

Les microplaques et les tubes reconditionn\u00e9s ont permis d’obtenir des courbes \u00e9talons comparables \u00e0 celles obtenues avec des consommables neufs, et ce, sur l’ensemble des cycles de reconditionnement. Pour les tubes, les valeurs de R\u00b2 sont rest\u00e9es syst\u00e9matiquement sup\u00e9rieures \u00e0 0.99, tant pour les tubes neufs que pour les tubes reconditionn\u00e9s, et aucune diff\u00e9rence statistiquement significative n’a \u00e9t\u00e9 d\u00e9tect\u00e9e au niveau de l’absorbance pour aucun des \u00e9talons. L\u2019absorbance \u00e0 blanc des tubes reconditionn\u00e9s \u00e9tait l\u00e9g\u00e8rement sup\u00e9rieure \u00e0 celle des tubes neufs (d\u2019environ 0.003 unit\u00e9 d\u2019absorbance), mais cette diff\u00e9rence n\u2019\u00e9tait pas statistiquement significative. Pour les microplaques, les valeurs de R\u00b2 sont rest\u00e9es sup\u00e9rieures \u00e0 0.97 dans toutes les conditions, et aucune diff\u00e9rence statistiquement significative n\u2019a \u00e9t\u00e9 d\u00e9tect\u00e9e au niveau de l\u2019absorbance pour aucun des \u00e9talons. <\/p>\n

Les tailles d’effet et les \u00e9carts-types ont l\u00e9g\u00e8rement vari\u00e9 d’un \u00e9talon \u00e0 l’autre et d’une r\u00e9p\u00e9tition \u00e0 l’autre dans les deux formats, mais n’ont pas montr\u00e9 de tendance constante en faveur de l’un ou l’autre groupe, ce qui sugg\u00e8re que la variation observ\u00e9e refl\u00e8te des facteurs exp\u00e9rimentaux normaux, tels que la pr\u00e9paration des \u00e9talons, leur manipulation ou le moment de la mesure, plut\u00f4t qu’un quelconque effet du reconditionnement.<\/p>\n

Ces r\u00e9sultats confirment que les microplaques et les tubes de microcentrifugeuse peuvent \u00eatre r\u00e9utilis\u00e9s en toute s\u00e9curit\u00e9 pour la quantification des prot\u00e9ines, sans perte de pr\u00e9cision ni de fiabilit\u00e9, et ce, sur cinq cycles de reconditionnement.[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

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