{"id":120405,"date":"2023-09-04T17:06:04","date_gmt":"2023-09-04T17:06:04","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2023\/09\/04\/presence-de-proteines\/"},"modified":"2024-09-09T16:00:03","modified_gmt":"2024-09-09T16:00:03","slug":"presence-de-proteines","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2023\/09\/04\/presence-de-proteines\/","title":{"rendered":"Pr\u00e9sence de prot\u00e9ines"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb605952″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb113011″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb205086″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Pr\u00e9sence de prot\u00e9ines<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb824723″]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb119296″ back_position=\u00a0\u00bbright top\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb746348″][vc_single_image media=\u00a0\u00bb119266″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb318485″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb511556″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb360120″][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb117630″]Microplaques reconditionn\u00e9es pour le dosage des prot\u00e9ines par l’essai \u00ab\u00a0Pierce BCA\u00a0\u00bb[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb160595″]Cette \u00e9tude a utilis\u00e9 des microplaques neuves et reconditionn\u00e9es pour un test Pierce\u2122 BCA Protein Assay. Deux microplaques \u00e0 96 puits ont \u00e9t\u00e9 contamin\u00e9es par 0,5 ml de BSA (2 mg\/ml), puis reconditionn\u00e9es selon notre proc\u00e9d\u00e9. Les standards de BSA ont \u00e9t\u00e9 pr\u00e9par\u00e9s selon les sch\u00e9mas de dilution sp\u00e9cifi\u00e9s dans le protocole<\/a> du kit Pierce\u2122 BCA Protein Assay (Thermo Fisher Scientific, num\u00e9ros de catalogue 23225, 23227 et A65453), avec des concentrations allant de 25 \u00b5g\/mL \u00e0 2000 \u00b5g\/mL.<\/p>\n

Chaque \u00e9chantillon a \u00e9t\u00e9 test\u00e9 en double sur des microplaques neuves et reconditionn\u00e9es conform\u00e9ment au protocole du kit. Pour l’essai, chaque standard de BSA ainsi que des contr\u00f4le negatifs (eau) ont \u00e9t\u00e9 pipet\u00e9s dans les puits d\u00e9sign\u00e9s. Les plaques ont \u00e9t\u00e9 incub\u00e9es pendant 2 heures \u00e0 37\u00b0C pour garantir la d\u00e9tection de faibles concentrations de prot\u00e9ines. Des courbes standard ont \u00e9t\u00e9 \u00e9tablies pour les microplaques neuves et reconditionn\u00e9es, afin de comparer et d’analyser leur absorbance. La courbe de la nouvelle microplaque a \u00e9galement \u00e9t\u00e9 utilis\u00e9e pour d\u00e9terminer les concentrations des contr\u00f4les n\u00e9gatifs (eau) sur les microplaques neuves et reconditionn\u00e9es et s’assurer de leur similitude.<\/p>\n

Cette exp\u00e9rience nous a permis d’\u00e9valuer la pr\u00e9sence de prot\u00e9ines r\u00e9siduelles.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb117024″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb214315″][vc_single_image media=\u00a0\u00bb120663″ media_width_percent=\u00a0\u00bb50″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb571862″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb985002″ shape_dividers=\u00a0\u00bb\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ radius=\u00a0\u00bbxl\u00a0\u00bb width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb672116″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb120655″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb100481″]Les courbes standards obtenues \u00e0 partir de microplaques neuves et reconditionn\u00e9es sont \u00e9quivalentes (les diff\u00e9rences observ\u00e9es se situent dans la fourchette autoris\u00e9e par le kit). En outre, les puits remplis d’eau dans les microplaques reconditionn\u00e9es n’ont montr\u00e9 aucune diff\u00e9rence par rapport aux puits de la nouvelle microplaque, ce qui indique qu’ils ne contenaient pas de prot\u00e9ines r\u00e9siduelles.<\/p>\n

De la m\u00eame mani\u00e8re, le kit a \u00e9t\u00e9 utilis\u00e9 pour analyser la pr\u00e9sence de prot\u00e9ines dans des tubes coniques de 1,5 mL. Aucune diff\u00e9rence n’a \u00e9t\u00e9 observ\u00e9e par rapport aux tubes neufs.[\/vc_icon][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ radius=\u00a0\u00bbxl\u00a0\u00bb width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb127811″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb120653″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbConclusion\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb508634″]Notre proc\u00e9d\u00e9 fonctionne de mani\u00e8re \u00e0 permettre l’utilisation de microplaques reconditionn\u00e9es et de tubes reconditionn\u00e9s dans des exp\u00e9riences de quantification pr\u00e9cise des prot\u00e9ines.[\/vc_icon][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb511556″][vc_column column_width_percent=\u00a0\u00bb100″ align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb360120″][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb816662″]\u00c9couvillons de prot\u00e9ines[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb3″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb100227″][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb153197″]Les indicateurs de nettoyage VERIFY\u00ae<\/sup> RESI-TEST\u2122<\/sup><\/a> d\u00e9tectent l’absence ou la pr\u00e9sence de prot\u00e9ines et de r\u00e9sidus prot\u00e9iques avec une sensibilit\u00e9 \u2265 1\u03bcg. Le test est colorim\u00e9trique : le bleu ou le vert fonc\u00e9 indique un \u00e9chantillon contamin\u00e9 par des prot\u00e9ines, et le brun ou le vert clair indique un \u00e9chantillon non contamin\u00e9 par des prot\u00e9ines. Ces tests sont utilis\u00e9s contr\u00f4ler l’efficacit\u00e9 de notre processus de nettoyage pour chaque lot de consommables reconditionn\u00e9s.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb117024″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb214315″][vc_single_image media=\u00a0\u00bb119197″ media_width_percent=\u00a0\u00bb100″ uncode_shortcode_id=\u00a0\u00bb356329″][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb0″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb541184″][vc_row_inner row_inner_height_percent=\u00a0\u00bb100″ back_color=\u00a0\u00bbcolor-nhtu\u00a0\u00bb overlay_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb30″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ limit_content=\u00a0\u00bb\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb200565″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb overlay_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column_inner column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb1″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb2″ style=\u00a0\u00bblight\u00a0\u00bb back_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb414397″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon icon_image=\u00a0\u00bb119198″ media_size=\u00a0\u00bb70″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbAvant reconditionnement\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb150119″]Les consommables pr\u00e9c\u00e9demment utilis\u00e9s pour manipuler des compos\u00e9s tels que du sang ou des cellules sont positifs pour les prot\u00e9ines.[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb2″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb2″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb168861″][vc_icon icon_image=\u00a0\u00bb119199″ media_size=\u00a0\u00bb50″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbApr\u00e8s reconditionnement\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb344728″]\u00c0 la fin de notre processus de nettoyage, les consommables sont test\u00e9s pour d\u00e9tecter la pr\u00e9sence de prot\u00e9ines avant d’\u00eatre emball\u00e9s. Un exemple est pr\u00e9sent\u00e9s ici pour des consommables pr\u00e9c\u00e9demment utilis\u00e9s avec du sang ou des cellules.[\/vc_icon][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

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