{"id":120405,"date":"2023-09-04T17:06:04","date_gmt":"2023-09-04T17:06:04","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2023\/09\/04\/presence-de-proteines\/"},"modified":"2026-08-26T19:19:44","modified_gmt":"2026-08-26T19:19:44","slug":"presence-de-proteines","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2023\/09\/04\/presence-de-proteines\/","title":{"rendered":"Quantification des prot\u00e9ines"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb2&Prime; bottom_padding=\u00a0\u00bb2&Prime; back_color=\u00a0\u00bbcolor-366045&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb605952&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb2\/3&Prime; uncode_shortcode_id=\u00a0\u00bb113011&Prime;][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1&Prime; text_size=\u00a0\u00bbh4&Prime; text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194&Prime; text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]<span class=\"font-100719 font-377187\">Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1&Prime; text_size=\u00a0\u00bbfontsize-155944&Prime; text_weight=\u00a0\u00bb600&Prime; text_space=\u00a0\u00bbfontspace-781688&Prime; uncode_shortcode_id=\u00a0\u00bb205086&Prime; text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]<span class=\"font-100719 font-377187\">Pr\u00e9sence de prot\u00e9ines<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1&Prime;][vc_button button_color=\u00a0\u00bbcolor-118531&Prime; radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0&Prime; link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb824723&Prime;]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; back_image=\u00a0\u00bb119642&Prime; back_position=\u00a0\u00bbright top\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/3&Prime; uncode_shortcode_id=\u00a0\u00bb746348&Prime;][vc_single_image media=\u00a0\u00bb119635&Prime; media_width_percent=\u00a0\u00bb100&Prime; alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb318485&Prime;][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb3&Prime; bottom_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb49&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb4&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; content_parallax=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb661626&Prime;][vc_column column_width_use_pixel=\u00a0\u00bbyes\u00a0\u00bb position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/1&Prime; column_width_pixel=\u00a0\u00bb900&Prime;][vc_custom_heading heading_semantic=\u00a0\u00bbh3&Prime; uncode_shortcode_id=\u00a0\u00bb388158&Prime;]Quantification des prot\u00e9ines pour une large gamme de concentrations[\/vc_custom_heading][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb179595&Prime;]<\/p>\n<p class=\"isSelectedEnd\" style=\"text-align: left;\">Le potentiel de r\u00e9utilisation des tubes dans des applications li\u00e9es aux prot\u00e9ines a \u00e9t\u00e9 \u00e9valu\u00e9 \u00e0 l&rsquo;aide de dosages quantitatifs de prot\u00e9ines sur une large plage de concentrations, allant de <strong>0,5 \u00e0 2 000 \u03bcg\/mL<\/strong>. Diff\u00e9rentes concentrations et diff\u00e9rentes m\u00e9thodes de d\u00e9tection ont \u00e9t\u00e9 \u00e9tudi\u00e9s afin d&rsquo;\u00e9valuer les performances tant \u00e0 de faibles qu&rsquo;\u00e0 de fortes concentrations en prot\u00e9ines. <\/p>\n<p class=\"isSelectedEnd\" style=\"text-align: left;\">Les r\u00e9sultats pr\u00e9sent\u00e9s ici comprennent :<\/p>\n<ul data-spread=\"false\">\n<li style=\"text-align: left;\"><strong>Dosage Pierce BCA dans des tubes de microcentrifugeuse :<\/strong> 25 \u00e0 2 000 \u03bcg\/mL<\/li>\n<li style=\"text-align: left;\"><strong>Dosage Pierce BCA dans des microplaques :<\/strong> 5 \u00e0 250 \u03bcg\/mL<\/li>\n<li style=\"text-align: left;\"><strong>Test de fluorescence AccuOrange dans des tubes de microcentrifugeuse :<\/strong> 0,5 \u00e0 1 \u03bcg\/mL<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner column_width_percent=\u00a0\u00bb100&Prime; align_horizontal=\u00a0\u00bbalign_right\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; align_mobile=\u00a0\u00bbalign_center_mobile\u00a0\u00bb mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime;][vc_button button_color=\u00a0\u00bbaccent\u00a0\u00bb border_width=\u00a0\u00bb0&Prime; link=\u00a0\u00bburl:%23BCA_result|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb170368&Prime;]R\u00e9sultats Pierce BCA[\/vc_button][\/vc_column_inner][vc_column_inner column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb4&Prime; align_mobile=\u00a0\u00bbalign_center_mobile\u00a0\u00bb mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime;][vc_button button_color=\u00a0\u00bbaccent\u00a0\u00bb border_width=\u00a0\u00bb0&Prime; link=\u00a0\u00bburl:%23Orange_results|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb101962&Prime;]R\u00e9sultats AccuOrange[\/vc_button][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb3&Prime; bottom_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; content_parallax=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb310156&Prime; el_id=\u00a0\u00bbBCA_result\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/1&Prime; uncode_shortcode_id=\u00a0\u00bb134650&Prime;][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb236418&Prime;]Microplaques et tubes reconditionn\u00e9s pour le dosage des prot\u00e9ines par la m\u00e9thode BCA de Pierce[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/1&Prime; uncode_shortcode_id=\u00a0\u00bb711417&Prime;][vc_column_text uncode_shortcode_id=\u00a0\u00bb135435&Prime;]<\/p>\n<p class=\"font-claude-response-body break-words whitespace-normal\">Afin de d\u00e9terminer si le reconditionnement influe sur la pr\u00e9cision de la quantification des prot\u00e9ines, nous avons r\u00e9alis\u00e9 le test BCA de Pierce selon deux m\u00e9thodes : une m\u00e9thode sur microplaque (plaques en polystyr\u00e8ne \u00e0 96 puits) et une m\u00e9thode sur tube (tubes de microcentrifugation en polypropyl\u00e8ne de 1,5 ml).<\/p>\n<p class=\"font-claude-response-body break-words whitespace-normal\">Trois microplaques et 18 tubes ont \u00e9t\u00e9 soumis \u00e0 cinq cycles de reconditionnement et r\u00e9utilis\u00e9s pour r\u00e9aliser le dosage apr\u00e8s chaque cycle, en comparaison avec des consommables neufs \u00e0 chaque cycle. Les deux formats ont suivi <a href=\"https:\/\/www.thermofisher.com\/document-connect\/document-connect.html?url=https:\/\/assets.thermofisher.com\/TFS-Assets%2FLSG%2Fmanuals%2FMAN0011430_Pierce_BCA_Protein_Asy_UG.pdf\">les protocoles<\/a> BCA standard, avec 9 \u00e9talons de concentration et un blanc \u00e0 l\u2019eau Milli-Q pour chaque condition (de 5 \u00e0 250 \u03bcg\/mL pour les plaques, et de 25 \u00e0 2 000 \u03bcg\/mL pour les tubes). L&rsquo;absorbance a \u00e9t\u00e9 mesur\u00e9e \u00e0 562 nm, et des courbes \u00e9talons ont \u00e9t\u00e9 \u00e9tablies pour chaque condition. L&rsquo;absorbance du blanc, l&rsquo;absorbance des courbes \u00e9talons et les \u00e9carts-types ont \u00e9t\u00e9 compar\u00e9s entre les consommables neufs et reconditionn\u00e9s afin d&rsquo;\u00e9valuer tout effet sur la pr\u00e9cision et la variabilit\u00e9 des mesures.   <\/p>\n<p class=\"font-claude-response-body break-words whitespace-normal\">Pour conna\u00eetre la m\u00e9thodologie compl\u00e8te, consultez notre \u00e9tude : <a class=\"underline underline underline-offset-2 decoration-1 decoration-current\/40 hover:decoration-current focus:decoration-current\" href=\"https:\/\/www.nature.com\/articles\/s44296-026-00108-9\">Mansouri et al., 2026 \u2014 <em>npj Materials Sustainability<\/em><\/a><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb0&Prime; bottom_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb117024&Prime;][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb-2&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime; mobile_height=\u00a0\u00bb300&Prime; uncode_shortcode_id=\u00a0\u00bb190872&Prime;][vc_single_image media=\u00a0\u00bb121331&Prime; media_width_percent=\u00a0\u00bb90&Prime; alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb687528&Prime;][vc_column_text text_lead=\u00a0\u00bbsmall\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb187262&Prime;]<span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\">Quantification des prot\u00e9ines <\/span><\/span><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">dans des microplaques \u00e0 96 puits <\/span><span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\">et des micro-tubes \u00e0 l&rsquo;aide du test BCA. L&rsquo;analyse statistique a \u00e9t\u00e9  <\/span><\/span><span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\"><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">r\u00e9alis\u00e9e \u00e0 l&rsquo;aide de la m\u00e9thode de Mann<\/span><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">\u2013<\/span><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">Whitney U avec correction de Bonferroni-Holm.  <\/span><\/span><\/span><span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\"><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">Aucune diff\u00e9rence statistiquement significative<\/span><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">n&rsquo;a \u00e9t\u00e9 observ\u00e9e. Chaque \u00e9talon a \u00e9t\u00e9 test\u00e9 en 5 <\/span> <span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">r\u00e9plicats<\/span> <\/span><\/span><span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\"><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">par plaque pour les microplaques et en trois r\u00e9plicats pour les tubes (soit un total de <\/span><\/span><\/span><span dir=\"ltr\" role=\"presentation\"><span class=\"current-selection\"><span class=\"current-selection\" dir=\"ltr\" role=\"presentation\">18 tubes).<\/span><\/span><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb121955&Prime;][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50&Prime; radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime; uncode_shortcode_id=\u00a0\u00bb872726&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon position=\u00a0\u00bbleft\u00a0\u00bb icon_image=\u00a0\u00bb120655&Prime; media_size=\u00a0\u00bb70&Prime; text_size=\u00a0\u00bbh2&Prime; align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb177936&Prime;][\/vc_icon][vc_column_text uncode_shortcode_id=\u00a0\u00bb157919&Prime;]<\/p>\n<p class=\"font-claude-response-body break-words whitespace-normal\">Les microplaques et les tubes reconditionn\u00e9s ont permis d&rsquo;obtenir des courbes \u00e9talons comparables \u00e0 celles obtenues avec des consommables neufs, et ce, sur l&rsquo;ensemble des cycles de reconditionnement. Pour les tubes, les valeurs de R\u00b2 sont rest\u00e9es syst\u00e9matiquement sup\u00e9rieures \u00e0 0.99, tant pour les tubes neufs que pour les tubes reconditionn\u00e9s, et aucune diff\u00e9rence statistiquement significative n&rsquo;a \u00e9t\u00e9 d\u00e9tect\u00e9e au niveau de l&rsquo;absorbance pour aucun des \u00e9talons. L\u2019absorbance \u00e0 blanc des tubes reconditionn\u00e9s \u00e9tait l\u00e9g\u00e8rement sup\u00e9rieure \u00e0 celle des tubes neufs (d\u2019environ 0.003 unit\u00e9 d\u2019absorbance), mais cette diff\u00e9rence n\u2019\u00e9tait pas statistiquement significative. Pour les microplaques, les valeurs de R\u00b2 sont rest\u00e9es sup\u00e9rieures \u00e0 0.97 dans toutes les conditions, et aucune diff\u00e9rence statistiquement significative n\u2019a \u00e9t\u00e9 d\u00e9tect\u00e9e au niveau de l\u2019absorbance pour aucun des \u00e9talons.   <\/p>\n<p class=\"font-claude-response-body break-words whitespace-normal\">Les tailles d&rsquo;effet et les \u00e9carts-types ont l\u00e9g\u00e8rement vari\u00e9 d&rsquo;un \u00e9talon \u00e0 l&rsquo;autre et d&rsquo;une r\u00e9p\u00e9tition \u00e0 l&rsquo;autre dans les deux formats, mais n&rsquo;ont pas montr\u00e9 de tendance constante en faveur de l&rsquo;un ou l&rsquo;autre groupe, ce qui sugg\u00e8re que la variation observ\u00e9e refl\u00e8te des facteurs exp\u00e9rimentaux normaux, tels que la pr\u00e9paration des \u00e9talons, leur manipulation ou le moment de la mesure, plut\u00f4t qu&rsquo;un quelconque effet du reconditionnement.<\/p>\n<p>Ces r\u00e9sultats confirment que les microplaques et les tubes de microcentrifugeuse peuvent \u00eatre r\u00e9utilis\u00e9s en toute s\u00e9curit\u00e9 pour la quantification des prot\u00e9ines, sans perte de pr\u00e9cision ni de fiabilit\u00e9, et ce, sur cinq cycles de reconditionnement.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb3&Prime; bottom_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; content_parallax=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb526631&Prime; el_id=\u00a0\u00bbOrange_results\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/1&Prime; uncode_shortcode_id=\u00a0\u00bb134650&Prime;][vc_custom_heading uncode_shortcode_id=\u00a0\u00bb102069&Prime;]Quantification des prot\u00e9ines par dosage fluorim\u00e9trique[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/1&Prime; uncode_shortcode_id=\u00a0\u00bb711417&Prime;][vc_column_text uncode_shortcode_id=\u00a0\u00bb849074&Prime;]<\/p>\n<p dir=\"ltr\">Afin de d\u00e9terminer si le reconditionnement influe sur la pr\u00e9cision de la quantification de faibles concentration de prot\u00e9ines, nous avons r\u00e9alis\u00e9 des dosages fluorim\u00e9triques de la prot\u00e9ine BSA (Bovine Serum Albumin) avec le kit AccuOrange (Biotium) sur des tubes de microcentrifugation en polypropyl\u00e8ne de 1,5 mL.<\/p>\n<p dir=\"ltr\">Des tubes de microcentrifugation ont \u00e9t\u00e9 soumis \u00e0 un cycle de reconditionnement puis r\u00e9utilis\u00e9s pour r\u00e9aliser les dosages, en comparaison avec des consommables neufs, pour trois marques (M1 Invitrogen Low-binding, M2 Corning, M3 FunChem). Cette exp\u00e9rience a suivi le <a href=\"https:\/\/biotium.com\/wp-content\/uploads\/2013\/07\/PI-30071.pdf\"><span style=\"font-weight: 400;\">protocole<\/span><\/a> standard, avec 5 \u00e9talons de concentration et un blanc \u00e0 l&rsquo;eau Milli-Q dans chaque condition. Chaque condition a \u00e9t\u00e9 mesur\u00e9e en trois r\u00e9plicats. Les valeurs de fluorescence des courbes d&rsquo;\u00e9talonnage et les coefficients de variation ont \u00e9t\u00e9 compar\u00e9s entre consommables neufs et reconditionn\u00e9s afin d&rsquo;\u00e9valuer tout effet sur la pr\u00e9cision et la variabilit\u00e9 des mesures.   <\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb0&Prime; bottom_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb117024&Prime;][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb-2&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime; mobile_height=\u00a0\u00bb300&Prime; uncode_shortcode_id=\u00a0\u00bb190872&Prime;][vc_single_image media=\u00a0\u00bb121440&Prime; media_width_percent=\u00a0\u00bb84&Prime; alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb178433&Prime;][vc_column_text text_lead=\u00a0\u00bbsmall\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb128562&Prime;]Quantification de la prot\u00e9ine BSA dans des tubes de microcentrifugation \u00e0 l&rsquo;aide d&rsquo;un test AccuOrange. Aucune diff\u00e9rence significative n&rsquo;a \u00e9t\u00e9 observ\u00e9e. Chaque concentration a \u00e9t\u00e9 test\u00e9e en 3 r\u00e9plicats par condition.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb121955&Prime;][vc_column column_width_percent=\u00a0\u00bb100&Prime; gutter_size=\u00a0\u00bb3&Prime; back_color=\u00a0\u00bbaccent\u00a0\u00bb overlay_alpha=\u00a0\u00bb50&Prime; radius=\u00a0\u00bbxl\u00a0\u00bb shift_x=\u00a0\u00bb0&Prime; shift_y=\u00a0\u00bb0&Prime; shift_y_down=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; medium_width=\u00a0\u00bb0&Prime; mobile_width=\u00a0\u00bb0&Prime; width=\u00a0\u00bb1\/2&Prime; uncode_shortcode_id=\u00a0\u00bb872726&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_icon position=\u00a0\u00bbleft\u00a0\u00bb icon_image=\u00a0\u00bb120655&Prime; media_size=\u00a0\u00bb70&Prime; text_size=\u00a0\u00bbh2&Prime; align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb177936&Prime;][\/vc_icon][vc_column_text uncode_shortcode_id=\u00a0\u00bb891554&Prime;]<\/p>\n<p dir=\"ltr\">Les tubes reconditionn\u00e9s ont permis d&rsquo;obtenir des courbes standard comparables \u00e0 celles obtenues avec des consommables neufs (Figure A). Les valeurs de R\u00b2 sont rest\u00e9es sup\u00e9rieures \u00e0 0,96 dans les deux conditions, tant pour les tubes neufs (R\u00b2 = 0,9604) que reconditionn\u00e9s (R\u00b2 = 0,9803). Aucune diff\u00e9rence significative marqu\u00e9e n&rsquo;a \u00e9t\u00e9 d\u00e9tect\u00e9e au niveau du signal pour aucune des concentrations (Figure B). La marque Low-binding (M1) n&rsquo;a montr\u00e9 aucun avantage d\u00e9tectable aux faibles concentrations.     <\/p>\n<p dir=\"ltr\">Les \u00e9carts et les coefficients de variation ont l\u00e9g\u00e8rement vari\u00e9 d&rsquo;un r\u00e9plicat \u00e0 l&rsquo;autre, mais sans tendance constante en faveur de l&rsquo;un ou l&rsquo;autre groupe, ce qui sugg\u00e8re que la variation observ\u00e9e refl\u00e8te des facteurs exp\u00e9rimentaux normaux comme la pr\u00e9paration des dilutions, le pipetage et le moment de la mesure, plut\u00f4t qu&rsquo;un effet du reconditionnement. Aux concentrations les plus basses, la variabilit\u00e9 est domin\u00e9e par le bruit instrumental \u00e0 l&rsquo;approche de la limite de d\u00e9tection du kit, et non par un ph\u00e9nom\u00e8ne d&rsquo;adsorption d\u00e9pendant de la concentration. <\/p>\n<p dir=\"ltr\">Ces r\u00e9sultats confirment que les tubes de microcentrifugation reconditionn\u00e9s peuvent \u00eatre r\u00e9utilis\u00e9s pour les manipulations de faibles concentrations de prot\u00e9ines, sans perte de pr\u00e9cision ni de fiabilit\u00e9.<\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row row_height_percent=\u00a0\u00bb0&Prime; override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2&Prime; top_padding=\u00a0\u00bb2&Prime; bottom_padding=\u00a0\u00bb2&Prime; back_color=\u00a0\u00bbcolor-366045&Prime; overlay_alpha=\u00a0\u00bb50&Prime; equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3&Prime; column_width_percent=\u00a0\u00bb100&Prime; shift_y=\u00a0\u00bb0&Prime; z_index=\u00a0\u00bb0&Prime; uncode_shortcode_id=\u00a0\u00bb605952&Prime; back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100&Prime; position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2&Prime; overlay_alpha=\u00a0\u00bb50&Prime; [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_wp_convertkit_post_meta":{"form":"-1","landing_page":"0","tag":"0","restrict_content":"0"},"footnotes":""},"categories":[166],"tags":[],"class_list":["post-120405","post","type-post","status-publish","format-standard","hentry","category-validation-fr"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/120405","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/comments?post=120405"}],"version-history":[{"count":39,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/120405\/revisions"}],"predecessor-version":[{"id":121475,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/120405\/revisions\/121475"}],"wp:attachment":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/media?parent=120405"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/categories?post=120405"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/tags?post=120405"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}