{"id":119665,"date":"2023-09-04T19:41:52","date_gmt":"2023-09-04T19:41:52","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2023\/09\/04\/mesures-dabsorbance\/"},"modified":"2024-09-09T16:11:16","modified_gmt":"2024-09-09T16:11:16","slug":"detection-de-molecules-non-specifiques","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2023\/09\/04\/detection-de-molecules-non-specifiques\/","title":{"rendered":"D\u00e9tection de mol\u00e9cules non sp\u00e9cifiques"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb183491″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb163685″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb989194″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb669511″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]D\u00e9tection de mol\u00e9cules non sp\u00e9cifiques[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb200253″]Sugg\u00e9rer des tests suppl\u00e9mentaires[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb119293″ back_position=\u00a0\u00bbcenter center\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb111384″][vc_single_image media=\u00a0\u00bb119266″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb748179″][\/vc_column][\/vc_row][vc_row][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb928223″]Les mesures d’absorbance effectu\u00e9es sur un spectrophotom\u00e8tre comprennent l’absorbance de toutes les mol\u00e9cules de l’\u00e9chantillon qui absorbent \u00e0 la longueur d’onde consid\u00e9r\u00e9e. Les acides nucl\u00e9iques pr\u00e9sentent un maximum d’absorbance \u00e0 260nm ; les prot\u00e9ines pr\u00e9sentent un maximum \u00e0 280nm ; l’absorption \u00e0 230nm peut \u00eatre caus\u00e9e par la contamination par certains compos\u00e9s organiques. Pour tester la pr\u00e9sence de mol\u00e9cules dans un tube \u00e0 essai, nous le remplissons d’eau Milli-Q, puis nous le vortexons pour extraire les r\u00e9sidus de toutes les surfaces possibles. Nous mesurons ensuite l’absorbance de la solution \u00e0 l’aide d’un lecteur de microplaques et la comparons \u00e0 celle de l’eau Milli-Q fra\u00eeche. Les r\u00e9sultats sont pr\u00e9sent\u00e9s ici pour l’A280 uniquement. De fa\u00e7on similaire, aucun contaminant n’a \u00e9t\u00e9 observ\u00e9 apr\u00e8s notre processus de reconditionnement pour A260 et A230.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb205994″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb162285″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_single_image media=\u00a0\u00bb119236″ media_width_percent=\u00a0\u00bb85″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb442970″][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb169706″][vc_row_inner row_inner_height_percent=\u00a0\u00bb100″ back_color=\u00a0\u00bbcolor-nhtu\u00a0\u00bb overlay_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb30″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ limit_content=\u00a0\u00bb\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb200565″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb overlay_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column_inner column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb2″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb2″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb141560″][vc_icon icon_image=\u00a0\u00bb119227″ media_size=\u00a0\u00bb60″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbAvant reconditionnement\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb158211″]Les consommables pr\u00e9c\u00e9demment utilis\u00e9s pour manipuler du sang ou des cellules conduisent \u00e0 une solution dont l’absorbance \u00e0 280, 260 et 230 nm est sup\u00e9rieure \u00e0 celle de l’eau milli-Q. Cela indique la pr\u00e9sence de contaminants.[\/vc_icon][\/vc_column_inner][vc_column_inner column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb1″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb2″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ mobile_height=\u00a0\u00bb300″ uncode_shortcode_id=\u00a0\u00bb878111″][vc_icon icon_image=\u00a0\u00bb119199″ media_size=\u00a0\u00bb60″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbApr\u00e8s reconditionnement\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb211331″]A la fin de notre processus de nettoyage, tous les consommables conduisent \u00e0 une solution ayant une valeur d’absorbance similaire \u00e0 celle de l’eau Milli-Q \u00e0 280, 260 et 230nm. Cela indique que notre processus de nettoyage est efficace pour se d\u00e9barrasser d’une vari\u00e9t\u00e9 de contaminants.[\/vc_icon][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

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