{"id":119664,"date":"2023-09-04T23:30:35","date_gmt":"2023-09-04T23:30:35","guid":{"rendered":"https:\/\/phoeniximpact.ca\/2023\/09\/04\/pcr\/"},"modified":"2024-09-09T21:00:49","modified_gmt":"2024-09-09T21:00:49","slug":"pcr","status":"publish","type":"post","link":"https:\/\/phoeniximpact.ca\/fr\/2023\/09\/04\/pcr\/","title":{"rendered":"PCR"},"content":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb194996″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb122944″][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbh4″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb460573″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]\u00c9tude de cas<\/span>[\/vc_custom_heading][vc_custom_heading text_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb heading_semantic=\u00a0\u00bbh1″ text_size=\u00a0\u00bbfontsize-155944″ text_weight=\u00a0\u00bb600″ text_space=\u00a0\u00bbfontspace-781688″ uncode_shortcode_id=\u00a0\u00bb159719″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]R\u00e9action en cha\u00eene par polym\u00e9rase<\/span>[\/vc_custom_heading][vc_empty_space empty_h=\u00a0\u00bb1″][vc_button button_color=\u00a0\u00bbcolor-118531″ radius=\u00a0\u00bbbtn-round\u00a0\u00bb outline=\u00a0\u00bbyes\u00a0\u00bb border_width=\u00a0\u00bb0″ link=\u00a0\u00bburl:https%3A%2F%2Fphoeniximpact.ca%2Ffr%2Fvalidation%2F%23contact|title:Contact|\u00a0\u00bb button_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb100595″]Recommandez une \u00e9tude de cas[\/vc_button][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_image=\u00a0\u00bb119299″ back_position=\u00a0\u00bbright bottom\u00a0\u00bb parallax=\u00a0\u00bbyes\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb142590″][vc_single_image media=\u00a0\u00bb119266″ media_width_percent=\u00a0\u00bb100″ alignment=\u00a0\u00bbright\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb168500″][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb3″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb90″ gutter_size=\u00a0\u00bb4″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb166449″][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ style=\u00a0\u00bbdark\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/1″ uncode_shortcode_id=\u00a0\u00bb155120″][vc_custom_heading text_color=\u00a0\u00bbcolor-118531″ heading_semantic=\u00a0\u00bbh3″ text_size=\u00a0\u00bbh3″ text_weight=\u00a0\u00bb700″ text_transform=\u00a0\u00bbuppercase\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb164289″ text_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb]Validation du processus de reconditionnement – tubes de 1,5 ml utilis\u00e9s pour la PCR[\/vc_custom_heading][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb208052″][vc_column width=\u00a0\u00bb1\/1″][vc_column_text text_lead=\u00a0\u00bbyes\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb623318″]Des tubes de 1,5 ml neufs et reconditionn\u00e9s ont \u00e9t\u00e9 utilis\u00e9s pour s’assurer qu’ils conservent leur int\u00e9grit\u00e9 tout au long du processus de reconditionnement.<\/p>\n

Le m\u00e9lange de r\u00e9action PCR pour pGFP-actine a \u00e9t\u00e9 pr\u00e9par\u00e9 dans 10 tubes diff\u00e9rents de 1,5 ml. Le contenu de 5 d’entre eux est ensuite transf\u00e9r\u00e9 dans 5 tubes PCR. Apr\u00e8s la PCR, un tampon de chargement est ajout\u00e9 aux tubes PCR et les \u00e9chantillons sont plac\u00e9s dans les premiers puits n\u00b02-6 d’un gel d’agarose.<\/p>\n

Les dix tubes de 1,5 ml sont nettoy\u00e9s et s\u00e9ch\u00e9s selon notre processus de reconditionnement. Cinq d’entre eux sont ensuite r\u00e9utilis\u00e9s pour pr\u00e9parer un m\u00e9lange de r\u00e9action PCR pour la pGFP-actine et les cinq autres sont utilis\u00e9s comme contr\u00f4les n\u00e9gatifs pour la m\u00eame r\u00e9action (contenant tous les r\u00e9actifs \u00e0 l’exception de la pGFP-actine). Leur contenu est ensuite transf\u00e9r\u00e9 dans 10 tubes PCR. Apr\u00e8s la PCR, un tampon de chargement est ajout\u00e9 aux tubes PCR et les \u00e9chantillons sont plac\u00e9s dans les puits n\u00b07-11 d’un gel d’agarose (processus avec pGFP-actine) et les puits n\u00b013-17 (contr\u00f4les n\u00e9gatifs avec des tubes reconditionn\u00e9s). Le puits n\u00b012 contient un contr\u00f4le n\u00e9gatif pr\u00e9par\u00e9 dans un nouveau tube de 1,5 ml et agit comme un v\u00e9ritable contr\u00f4le n\u00e9gatif. Le puits n\u00b01 contient le marqueur de poids mol\u00e9culaire.<\/p>\n

Cette exp\u00e9rience nous a permis de tester la pr\u00e9sence d’ADN r\u00e9siduel.[\/vc_column_text][\/vc_column][\/vc_row][vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb0″ bottom_padding=\u00a0\u00bb0″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb140493″][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb2″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/3″ uncode_shortcode_id=\u00a0\u00bb273193″][vc_row_inner row_inner_height_percent=\u00a0\u00bb100″ back_color=\u00a0\u00bbcolor-nhtu\u00a0\u00bb overlay_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb30″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ limit_content=\u00a0\u00bb\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb200565″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb overlay_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column_inner column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb3″ style=\u00a0\u00bblight\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb0″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb4″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb1\/2″ uncode_shortcode_id=\u00a0\u00bb342301″][vc_icon icon_image=\u00a0\u00bb119199″ media_size=\u00a0\u00bb60″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbR\u00e9sultats\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb235200″]Tous les tubes reconditionn\u00e9s n’ont produit aucun signal dans leurs puits respectifs, ce qui indique qu’ils ne contenaient pas d’ADN r\u00e9siduel.[\/vc_icon][vc_icon icon_image=\u00a0\u00bb119144″ media_size=\u00a0\u00bb60″ text_size=\u00a0\u00bbh2″ text_reduced=\u00a0\u00bbyes\u00a0\u00bb align=\u00a0\u00bbleft\u00a0\u00bb title=\u00a0\u00bbConclusion\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb981500″]Notre processus de reconditionnement a fonctionn\u00e9 de mani\u00e8re \u00e0 ce que les consommables lav\u00e9s et s\u00e9ch\u00e9s puissent \u00eatre utilis\u00e9s plusieurs fois dans le d\u00e9partement de biologie mol\u00e9culaire.[\/vc_icon][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column column_width_percent=\u00a0\u00bb100″ gutter_size=\u00a0\u00bb3″ override_padding=\u00a0\u00bbyes\u00a0\u00bb column_padding=\u00a0\u00bb0″ back_color=\u00a0\u00bbcolor-xsdn\u00a0\u00bb overlay_alpha=\u00a0\u00bb50″ shift_x=\u00a0\u00bb0″ shift_y=\u00a0\u00bb-2″ shift_y_down=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ medium_width=\u00a0\u00bb0″ mobile_width=\u00a0\u00bb0″ width=\u00a0\u00bb2\/3″ uncode_shortcode_id=\u00a0\u00bb830339″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_single_image media=\u00a0\u00bb119247″ media_width_percent=\u00a0\u00bb80″ alignment=\u00a0\u00bbcenter\u00a0\u00bb uncode_shortcode_id=\u00a0\u00bb736068″][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row row_height_percent=\u00a0\u00bb0″ override_padding=\u00a0\u00bbyes\u00a0\u00bb h_padding=\u00a0\u00bb2″ top_padding=\u00a0\u00bb2″ bottom_padding=\u00a0\u00bb2″ back_color=\u00a0\u00bbcolor-366045″ overlay_alpha=\u00a0\u00bb50″ equal_height=\u00a0\u00bbyes\u00a0\u00bb gutter_size=\u00a0\u00bb3″ column_width_percent=\u00a0\u00bb100″ shift_y=\u00a0\u00bb0″ z_index=\u00a0\u00bb0″ uncode_shortcode_id=\u00a0\u00bb194996″ back_color_type=\u00a0\u00bbuncode-palette\u00a0\u00bb][vc_column column_width_percent=\u00a0\u00bb100″ position_vertical=\u00a0\u00bbmiddle\u00a0\u00bb align_horizontal=\u00a0\u00bbalign_center\u00a0\u00bb gutter_size=\u00a0\u00bb2″ overlay_alpha=\u00a0\u00bb50″ […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[167],"tags":[],"class_list":["post-119664","post","type-post","status-publish","format-standard","hentry","category-etude-de-cas"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/119664","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/comments?post=119664"}],"version-history":[{"count":2,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/119664\/revisions"}],"predecessor-version":[{"id":120996,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/posts\/119664\/revisions\/120996"}],"wp:attachment":[{"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/media?parent=119664"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/categories?post=119664"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phoeniximpact.ca\/fr\/wp-json\/wp\/v2\/tags?post=119664"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}